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KMID : 0378019690120010065
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New Medical Journal
1969 Volume.12 No. 1 p.65 ~ p.74
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Influeces of the Cultural Environments on the Pattern of Biochemical Reactions of Bacterial Species.
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Abstract
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Biochemical tests are one of the most important studies in determining bacterial species. Since the metabolic activities of bacteria are influenced by the physico-chemical natures of environment, results of biochemical reactions, which are used as the methods of identification and classification of bacteria, .might be different when the cultural conditions differ.
Although it has been presumed that the biochemical activities of bacteria in host tissues determine their virulence, it is, at present, not possible to correlate the knowledge of bacterial physiology and diseae procesees. This is, at least, partly due to the fact that it has not been possible to study the bacterial physiology under the conditions which are exact duplicates of in vivo surrounding.
However, it was reported that studies, performed under the conditions partly similar with the ones which are supposed to prevail in vivo, might help in under-standing the pathogenesis of Ps. aeruginosa.
Thus, the author undertook the studies on biochemical reactions of bacteria under the varied cultural conditions for some of the stock strains of Department of Microbiology, College of Medicine, SNU, which include Staph. aureus, Staph. albus, Es. co´li, Kl. aerogenes, Sal. typhosa, Sal. paratyphi A, Sal. paratyphi B. Sh. dysenteriae, Sh. flexneri, Sh. bovdii, Proteus vulgaris, Proteus mirabilis and Ps. -aeruginosa.
The organisms were grown aerobically at 23C. and 37C. and in candle-jar at 40C. Their sugar fermentation and IMVC reactions were studied.
The results might be summarized as follows:
1. The st rains of Es. coli, Kl. aerognenes, Sal. enteritidis, mirabilis, and Ps. aerugmosa did not show any significant changes in the pattern of biochemical reactions under the varied cultural coalitions studied.
2. The strains of Staph. aureus, Staph. albus, Sal. typhosa, Sal. paratyphi A, Sal. paratyphi B. Sh. dysenteriae, Sh. flexneri, Sh. boydii, and P. vulgaris showed marked differences in the results of biochemical tests when the cultural conditions differed.
3. The biochemical activities of the bacterial species appeared generally more active at 40C. in candlejar than under aerobic condition at 37℃
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